PLC activation in wild-type andlyn^−/−platelets.

Phosphorylation of pleckstrin and formation of phosphatidic acid were measured over time from 0 to 180 seconds after the addition of an agonist in platelets treated with 1 mmol/L EGTA and 10 μmol/L indomethacin. The autoradiographs show pleckstrin phosphorylation (A) and phosphatidic acid production (B) stimulated by CRP (1.0 μg/mL) and thrombin (0.1 U/mL) in wild-type and lyn^−/−platelets compared with wild type. Results are representative of 4 experiments.