Fig. 2.
Viability and proliferation of 32Dc13 cells expressing wild-type BCR/ABL, p185ΔBCR, or both p185ΔBCR and Bcl-2.
(A) Numbers of viable cells (triplicate counts) were determined by trypan blue exclusion at the indicated times after removal of IL-3. Results are representative of 3 independent experiments using 3 p185ΔBCR/Bcl-2 clones. Cells (105/mL) were plated in 25-mL flasks (B) with or (C) without IL-3–containing conditioned medium and counted by trypan blue exclusion at the indicated times. Results are representative of 3 independent experiments using 3 individual clones.