American Society of Hematology

Fig. 4.

$Fig. 4. Expression of mitochondrial Raf-1 in BCR/ABL and p185ΔBCR/Bcl-2–coexpressing cells. / (A) Levels of HSP90 and subunit IV of COX were measured as control of loading and subcellular fractionation. (B) Phosphorylation of BAD in BCR/ABL (wild-type and mutant) and p185ΔBCR/Bcl-2–coexpressing cells. Cells were starved of both IL-3 and serum for 3 hours and then cultured in 32P-orthophosphate–containing medium for 3 hours. Cell lysates were immunoprecipitated with anti-BAD polyclonal antibody, and phosphorylation was assessed by autoradiography.$

Expression of mitochondrial Raf-1 in BCR/ABL and p185ΔBCR/Bcl-2–coexpressing cells.

(A) Levels of HSP90 and subunit IV of COX were measured as control of loading and subcellular fractionation. (B) Phosphorylation of BAD in BCR/ABL (wild-type and mutant) and p185ΔBCR/Bcl-2–coexpressing cells. Cells were starved of both IL-3 and serum for 3 hours and then cultured in ³²P-orthophosphate–containing medium for 3 hours. Cell lysates were immunoprecipitated with anti-BAD polyclonal antibody, and phosphorylation was assessed by autoradiography.

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