Proliferation and leukemogenic potential of p185ΔBCR/Bcl-2–coexpressing 32Dc13 cells isolated from the spleen of leukemic mice.
(A) p185ΔBCR/Bcl-2 cells maintained in IL-3–free medium and cells recovered from the spleen of primary recipient leukemic mice (p185ΔBCR/Bcl-2 S) were plated at a density of 105 cells per mL in 25-mL flasks in the absence of IL-3–containing medium and counted by trypan blue exclusion at the indicated times. Results are representative of 3 independent experiments using 3 individual clones. (B) Survival of SCID mice injected with p185ΔBCR/Bcl-2 or p185ΔBCR/Bcl-2 S cells. Mice were injected intravenously with 5 × 106 cells. Eight mice were included in the p185ΔBCR/Bcl-2 group (1 clone), and 15 mice were included in the p185ΔBCR/Bcl-2 S group (5 mice for each of 3 clones).
