Colony assay for BFU-E and for MKs after cell sorting at day 4 or day 7.

The cells were sorted at day 4 using anti–CD41-PE and anti-GPIbα–Alexa 488, and at day 7 with an anti–CD41-PE and an anti-GPV revealed by an Alexa 488–GAM. ▪, CD41⁺GPIbα⁺; ░, CD41⁺GPIbα⁺; ▨, CD41⁺GPV⁻; ▩, CD41⁺GPV⁺. (A) The percentage of positive cells represents the proportion of BFU-E colonies compared to the total number of colonies obtained in methylcellulose medium. (B) Number of megakaryocytic colonies obtained in collagen medium.