Fig. 2.
![Fig. 2. Northern blot analysis. / Ten micrograms of total RNA sample were electrophoresed on agarose formaldehyde gels and transferred onto nylon membranes. The blots were hybridized with [α-32P] dCTP-labeled cDNA probes at 65°C overnight using a standard method. Filters were rinsed to a final stringency of 0.25 × SSC and exposed to a Kodak X-Omat or a Biomax film at -70 °C with an intensifying screen. After sequential stripping of the probes by a standard method, the blot was rehybridized with a GAPDH probe as a control.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/12/10.1182_blood.v96.12.3953/5/h82200348002.jpeg?Expires=1735509085&Signature=Sn8qbTLx5RUnCt62LrS2N37g2acx2JkaBEGWvR8NzIlcDetTZp4zBNKoHsExcqTVqBir93MbhxOlE1aJ4S9TWYXuEDGS8dN6kIzhjdHzOkxSggE0Yi~~bztGLXZmLusrmflWdtcw~lnXkwHcJCcSWIgBofS~xt9xLH6DtsvVHlv19bj5nRJjpjPJ1e6VtqQ7Pj30kxuMOD8kO1xMrVbYFKA~9~Lea20Bp-VtULo99But6SobYHuBziq9If6RkfAFEl41g9jiDupeld4iZzD~JE~DOKm0Uvrfghjhy4cMkT8jMnl1ljhFxkC8LmHevX7e1OpWLBA~znKCmoyBQ9CwlQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Northern blot analysis.
Ten micrograms of total RNA sample were electrophoresed on agarose formaldehyde gels and transferred onto nylon membranes. The blots were hybridized with [α-32P] dCTP-labeled cDNA probes at 65°C overnight using a standard method. Filters were rinsed to a final stringency of 0.25 × SSC and exposed to a Kodak X-Omat or a Biomax film at -70 °C with an intensifying screen. After sequential stripping of the probes by a standard method, the blot was rehybridized with a GAPDH probe as a control.
