SLF-stimulated tyrosine phosphorylation of PLC-γ.

(A) Expression of c-Kit on 32D-kitWT and KitYF728 cells were compared with BMMCs. Cells were incubated with anti-cKit antibody 2B8 and analyzed by flow cytometry. The shaded histogram represents BMMCs with secondary antibody alone. Both 32D infectants exhibited a similar histogram when stained with secondary alone. (B) PLC-γ1 from BMMCs, 32D-KitWT, or 32D-KitYF728 cells stimulated for 5 minutes with sSLF was immunoprecipitated, resolved by SDS-PAGE, transferred to nitrocellulose, and blotted with antiphosphotyrosine antibodies (upper panel). The blot was then stripped and reprobed with anti-PLCγ1 antisera. This experiment was repeated 3 times with similar results.