Myelopoietic and B-lymphopoietic capacity of buffy coat and hematon in LTC.

TNC and CFU were determined from the nonadherent (NA) and adherent cell fractions (AD). Buffy coat (A) and hematon (B) cultures. Commutation from myelopoiesis (Dexter conditions) to B lymphopoiesis (Whitlock-Witte conditions) in LTC was measured by analyzing the GM-CFU and B220⁺ cell populations. TNC-NA (○), TNC-AD (●), GM-CFU-NA (■), GM-CFU-AD (▪), HPP-CFC-NA (▵), HPP-CFC-AD (▴), B220⁺ B lymphocytes (*). Representative experiments repeated 3 times with similar results are shown. Data points represent the mean (±1 SEM) of 4 determinations.