Fig. 7.
Stromal and hematopoietic cell composition of the hematon core complex.
The hematon core complex fraction was purified from young adult mice, washed in PBS/0.5% BSA/0.1% Na azide, and cytocentrifuged. Immunostaining of native preparations with antibodies to Stro-1, desmin, Flk-1, Sca-1, VLA-4, CD34, Thy-1, c-kit, CD41, and acetylcholinesterase reaction on adherent core complexes at day 4 in culture show the discrete localization of cells within the hematon core complex or in close contact with the vascular endothelium.