Colony formation of quail bone marrow cells in MeC.
HPC-enriched bone marrow from ED14 quail embryos was incubated in MeC for 6 days, prior to colony counting. The numbers of colonies per 5 × 104 seeded HPCs per culture are indicated on the y-axis. These colonies numbers were tabulated according to both cell phenotype and size, with gray and black bars denoting colonies of 20 to 50 cells and more than 50 cells, respectively. The results are presented as mean values for 5 separate experiments, with the error bars corresponding to the SD of total numbers for each colony category. The categories indicated on the x-axis are for colonies of erythroid (E), granulocyte/macrophage (GM), granulocyte/erythroid/macrophage (GEM), granulocyte (G), macrophage/erythroid (ME), and macrophage (M) lineage cells.
