Fig. 3.
Blood cell phenotypes exhibited by avian bone marrow.
Bone marrow–derived HPCs were cultured for 6 days in either MeC (A, C, D, E, I) or FB (B, F-H) gels and subsequently stained with MGG solution. Representative cell types that developed from the bone marrow cultures were as follows: clustered vacuolated macrophages surrounded by erythroblasts (A), colony-containing erythroblasts (arrowheads) and developing erythrocytes (arrows) (B), mature RBCs (C), aggregated macrophages (D), mixed areas containing macrophages (asterisk), monocytes (arrows) and heterophils (arrowhead) (E), heterophils (note granules; arrow) (F), basophils (G), eosinophils (H), and thrombocytes (I). All the cell phenotypes shown were equally prevalent in both MeC and FB cultures. Scale bar: A, B, 13 μm; C, H, 8 μm; D, G, I, 14 μm; E, 20 μm; F, 10 μm.