CD34⁺ cells from different hematopoietic origins are efficiently transduced by the TRIP vector.

(A) Comparison of EGFP expression in purified CD34⁺ cells from CB, adult PBMC, and BM cells transduced in parallel using a TRIP vector concentration of 100 ng P24/mL. Dot-plot analyses demonstrating EGFP and CD34 expression are shown. (B) EGFP expression in identically transduced CD34⁺ and CD34⁺CD38⁻cells isolated from the same CB sample. Percentages of gated cells are indicated in each quadrant.