Fig. 6.
Fig. 6. Identification of ELL sequences required for immortalization of primary murine myeloid progenitors. / (A) Thick lines under the schematic of ELL indicate sequences fused to MLL5′; horizontal bars indicate the number of colonies generated per 104 cells plated in third-round cultures. Bars represent the mean ± SD of 3 independent experiments. Retroviral transduction efficiency for the various constructs was 60% (MLL-5′), 39% (MLL-ELL), 34% (+R1), 33% (Δ150-200), 29% (Δ374-620), 27% (C1), and 43% (C2). (B) Western blot analysis of fusion protein expression in transiently transfected φNX cells. Lane 1, MSCV vector; lane 2, MLL5′; lane 3, MLL-ELL; lane 4, MLL-ELLΔ150-200; lane 5, MLL-ELLΔ374-620; lane 6, MLL-ELLC1; lane 7, MLL-ELLC2.

Identification of ELL sequences required for immortalization of primary murine myeloid progenitors.

(A) Thick lines under the schematic of ELL indicate sequences fused to MLL5′; horizontal bars indicate the number of colonies generated per 104 cells plated in third-round cultures. Bars represent the mean ± SD of 3 independent experiments. Retroviral transduction efficiency for the various constructs was 60% (MLL-5′), 39% (MLL-ELL), 34% (+R1), 33% (Δ150-200), 29% (Δ374-620), 27% (C1), and 43% (C2). (B) Western blot analysis of fusion protein expression in transiently transfected φNX cells. Lane 1, MSCV vector; lane 2, MLL5′; lane 3, MLL-ELL; lane 4, MLL-ELLΔ150-200; lane 5, MLL-ELLΔ374-620; lane 6, MLL-ELLC1; lane 7, MLL-ELLC2.

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