Endothelial cell proliferation in the presence and absence of VEGF, FGF-2, and fibrinogen.

Endothelial cells were plated on gelatin-coated wells in McCoy 5A medium supplemented with 20% FBS, 50 μg/mL ECGS, and 100 μg/mL heparin and allowed to adhere for 6 hours. The cells were then washed twice with McCoy medium and incubated in serum-free medium containing 1% Nutridoma, 0.037 MBq/mL (1 μCi/mL) ³H-thymidine with or without 20 ng/mL VEGF, 25 ng/mL FGF-2, and 10 μg/mL fibrinogen (FBG) for 24 hours. Isotope incorporated into DNA was precipitated with TCA, collected by vacuum filtration, and measured by scintillation counting. Results are shown as mean ± SD of 4 different experiments.