Endothelial cell proliferation on fibrinogen- or fibrin-coated surfaces in the presence and absence of VEGF and FGF-2.

Endothelial cells were plated in wells coated with fibrinogen (filled bars) or fibrin (open bars) in the absence or presence of 20 ng/mL VEGF, 25 ng/mL FGF-2, or both. After 6 hours the medium was removed and replaced with serum-free medium containing 0.037 MBq/mL (1 μCi/mL)³H-thymidine, and the cultures were incubated for an additional 24 hours. No growth factor was present in the medium. Isotope incorporated into DNA was precipitated with TCA, collected by vacuum filtration, and measured by scintillation counting. Results are shown as mean ± SD of 3 different experiments.