Fig. 2.
Tissue specificity of sPLA2-induced EC migration.
BAECs, HMVECs, and BPAECs were placed in the upper compartment of a Transwell chamber, and bvPLA2 (1 μmol/L) was placed in the lower compartment. Migration was allowed to occur for 2 hours at 37°C and 5% CO2. Filters were removed and nonmigrated cells were scraped off. Filters were stained using Diff-Quick. Nuclei from 4 random power fields (× 10)/filter were counted. Bars indicate the mean ± SE of 3 independent experiments. Asterisk indicates statistical significance (P < .01 versus basal migration).
