PCR amplification of vessel wall tissue.

Sections containing affected testicular arteries were microdissected to remove vessel wall cells. H&E stain of section before dissection (A) and after dissection (C) show that portions of the vessel were removed. DNA was prepared from the removed sections labeled 1 and 2 (B) and subjected to EBV-specific amplification by PCR (D). The amplified products were visualized by gel electrophoresis and ethidium bromide staining. Lanes 1 and 2, vessel wall dissections as labeled in panel B; lane 3, amplification of an EBV-positive lymphoblastoid cell line; lane 4, seminiferous tubule; lane 5, negative control; and lane 7, 1 ng EBV-positive genome.