Cotransfection of an HNF4 expression vector alters expression of reporter gene from vectors with WT and MT55 fVII promoter sequence.

A total of 3 μg of WT or MT55 reporter vectors were transiently transfected into HepG2 cells, with or without 6 μg of the pCDNAI-HNF4 expression vector or pUC-19 plasmid DNA, as noted. The growth hormone values were corrected for expression from the promoterless pOGH vector under parallel conditions, and for transfection efficiency, then normalized to expression from the WT vector in the absence of coexpressed HNF4. The results shown (3.97% ± 2.87% [1 SD] for MT55 plasmid versus 100% ± 13.1% [1 SD] for WT plasmid, in the absence of pCDNAI-HNF4; 187% ± 99% [1 SD] for MT55 plasmid versus 610% ± 185% [1 SD] for WT plasmid, in the presence of pCDNAI-HNF4) were the averages from 3 experiments. The total number of replicates in each group is shown.