CD30 mediates the reduction of TRAF2 in ALCL and HD cells.

(A) Michel and L428 cells were incubated with the immobilized CD30 agonistic antibodies M67 or isotype control antibodies. Cells were harvested 12 hours later and standardized for protein levels, and TRAF2 and β-tubulin were detected by immunoblot analysis as described in “Materials and methods.” Michel lysates (16 μg) and L428 lysates (26 μg) were loaded in the indicated lanes. (B) Michel cells were preincubated with either a media control or ZVAD-fmk (50 μmol/L) before incubation with the immobilized CD30 agonistic antibodies M67 or isotype control antibodies. Cells were harvested 12 hours later and standardized for protein levels, and TRAF2 and β-tubulin were detected by immunoblot analysis as described in “Materials and methods.” Michel lysates (12.3 μg) were loaded in the indicated lanes.