Fig. 1.
Fig. 1. Schematic representation. / Proviral forms of SFCMM3 (A) and G1Tk1SvNa (B) vectors used for the transduction of CEM cells and primary T lymphocytes. LTR indicates long terminal repeats derived from Moloney murine leukemia virus (MLV) and Moloney sarcoma virus (MSV); HSV-Tk, herpes simplex virus gene sequence; SV40, simian virus 40 early promoter; ΔLNGFR, low-affinity nerve growth factor receptor cDNA truncated in the cytoplasmic domain; NeoR, neomycin phosphotransferase cDNA. Restriction enzyme sites for EcoRI and SacI used for the digestion of genomic DNAs derived from the CEM-Tk clones are indicated. The Tk1 and NGF probes were used to identify the provirus sequences in the genomic DNA. The Tk2 probe was also used to confirm the specificity of PCR products. The location of the PCR primers designed to amplify the SFCMM3 and G1Tk1SvNa provirus sequences are shown, together with the sizes of the PCR products.

Schematic representation.

Proviral forms of SFCMM3 (A) and G1Tk1SvNa (B) vectors used for the transduction of CEM cells and primary T lymphocytes. LTR indicates long terminal repeats derived from Moloney murine leukemia virus (MLV) and Moloney sarcoma virus (MSV); HSV-Tk, herpes simplex virus gene sequence; SV40, simian virus 40 early promoter; ΔLNGFR, low-affinity nerve growth factor receptor cDNA truncated in the cytoplasmic domain; NeoR, neomycin phosphotransferase cDNA. Restriction enzyme sites for EcoRI and SacI used for the digestion of genomic DNAs derived from the CEM-Tk clones are indicated. The Tk1 and NGF probes were used to identify the provirus sequences in the genomic DNA. The Tk2 probe was also used to confirm the specificity of PCR products. The location of the PCR primers designed to amplify the SFCMM3 and G1Tk1SvNa provirus sequences are shown, together with the sizes of the PCR products.

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