SDF-1, in contrast to TPO, does not affect the survival of normal human α_IIbβ₃⁺ cells but activates calcium flux.

(A) Annexin V binding to human α_IIbβ₃⁺ cells cultured for 12 hours in the absence of growth factors (i), in the presence of 100 ng/mL TPO (ii), or in the presence of 500 ng/mL SDF-1 (iii). A representative experiment of 3 is demonstrated. (B) Detection of activated caspase-3 in normal human α_IIbβ₃⁺ cells cultured in the presence of TPO, TPO + LY294002 (10 μmol/L), and TPO + LY294002 (30 μmol/L). A representative experiment of 3 is demonstrated. (C) Detection of PARP cleavage in normal human α_IIbβ₃⁺ cells cultured in the presence of TPO (lane 1), TPO + DMSO (lane 2), and TPO + LY294002 (30 μmol/L) (lane 3). A representative experiment of 3 is demonstrated.