Fig. 3.
SDF-1 and TPO stimulate adhesion of normal human αIIbβ3+ cells to fibrinogen and vitronectin.
Adhesion was measured to BSA- (control), fibrinogen-, and vitronectin-coated microtiter plates. Cells in the adhesion assay were nonstimulated (control) or stimulated with 500 ng/mL SDF-1, 100 ng/mL TPO, 500 ng SDF-1 + 100 ng TPO, 2 U/mL thrombin, or 100 ng/mL IL-3. The number of cells stuck to the plates was quantified by colorimetric phosphatase assay. Y axis: OD values at 405 nm. Experiments were repeated 3 times in triplicate, yielding similar results. * and **P < .0001 compared to control. ^ and ^^P < .0001 compared to cells untreated with LY294002.
