Fig. 5.
Fig. 5. SDF-1 and TPO activate MAPK (p42/44) and p90 RSK in normal human αIIbβ3+ cells. / Western blot analysis of MAPK phosphorylation (A) and p90 RSK (B) in normal human αIIbβ3+ cells. Cells were nonstimulated (lane 1) or stimulated with 100 ng/mL TPO (lanes 2, 4, 6, 8) or 500 ng/mL SDF-1 (lane 3, 5, 7, 9) for 1 minute (lanes 2, 3), 10 minutes (lanes 4, 5), 30 minutes (lanes 6, 7), and 1 hour (lanes 8, 9). Equal loading in the lanes was evaluated by stripping the blot and reprobing with an anti-MAPK (p42/44) antibody or anti-p90 RSK antibody. (C) Densitometric data showing changes in MAPK p42/44 phosphorylation. The experiment was repeated 5 times for MAPK p42/44 and 3 times for p90 RSK with similar results. Data are presented from 5 independent experiments.

SDF-1 and TPO activate MAPK (p42/44) and p90 RSK in normal human αIIbβ3+ cells.

Western blot analysis of MAPK phosphorylation (A) and p90 RSK (B) in normal human αIIbβ3+ cells. Cells were nonstimulated (lane 1) or stimulated with 100 ng/mL TPO (lanes 2, 4, 6, 8) or 500 ng/mL SDF-1 (lane 3, 5, 7, 9) for 1 minute (lanes 2, 3), 10 minutes (lanes 4, 5), 30 minutes (lanes 6, 7), and 1 hour (lanes 8, 9). Equal loading in the lanes was evaluated by stripping the blot and reprobing with an anti-MAPK (p42/44) antibody or anti-p90 RSK antibody. (C) Densitometric data showing changes in MAPK p42/44 phosphorylation. The experiment was repeated 5 times for MAPK p42/44 and 3 times for p90 RSK with similar results. Data are presented from 5 independent experiments.

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