Fig. 10.
Fig. 10. SDF-1, in contrast to TPO, does not phosphorylate STAT-1, STAT-5, or STAT-6 in normal human αIIbβ3+ cells. / Western blot analysis of STAT-1 phosphorylation (A), STAT-5 phosphorylation (B), and STAT-6 phosphorylation (C) in normal human αIIbβ3+ cells. Cells were nonstimulated (lane 1) or stimulated with 100 ng/mL TPO (lanes 2, 4, 6, 8) or 500 ng/mL SDF-1 (lanes 3, 5, 7, 9) for 1 minute (lanes 2, 3),10 minutes (lanes 4, 5), 30 minutes (lanes 6, 7), and 1 hour (lanes 8, 9). Equal loading in the lanes was evaluated by stripping the blot and reprobing with an anti–STAT-1, anti–STAT-5, or anti–STAT-6 antibody. The experiment was repeated 3 times with similar results.

SDF-1, in contrast to TPO, does not phosphorylate STAT-1, STAT-5, or STAT-6 in normal human αIIbβ3+ cells.

Western blot analysis of STAT-1 phosphorylation (A), STAT-5 phosphorylation (B), and STAT-6 phosphorylation (C) in normal human αIIbβ3+ cells. Cells were nonstimulated (lane 1) or stimulated with 100 ng/mL TPO (lanes 2, 4, 6, 8) or 500 ng/mL SDF-1 (lanes 3, 5, 7, 9) for 1 minute (lanes 2, 3),10 minutes (lanes 4, 5), 30 minutes (lanes 6, 7), and 1 hour (lanes 8, 9). Equal loading in the lanes was evaluated by stripping the blot and reprobing with an anti–STAT-1, anti–STAT-5, or anti–STAT-6 antibody. The experiment was repeated 3 times with similar results.

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