Effect of I-309 on HUVEC chemotaxis and invasion.

(A) Chemotaxis was assayed using the 48-well microchamber. Results are expressed as the migration index ± SEM of 8 experiments performed in triplicate using a range of I-309 concentrations. (B) Treatment with PTX (5 ng/mL) selectively inhibited I-309– but not FGF-2–induced chemotaxis (used at 30 ng/mL and 20 ng/mL, respectively). Shown is the percentage migration of untreated versus PTX-treated HUVECs of an average of 3 experiments performed in triplicate. (C) HUVEC invasion was evaluated with the 48-well microchamber. Shown is a representative experiment of 4 performed in triplicate. The results are expressed as the average number of cells per high-power field ± SD of 6 counted.