Neovascularization induced by I-309 in the CAM.

(A) CAM of 12-day-old chick embryo incubated for 4 days with a sponge adsorbed with 400 ng I-309. Note the presence of an increased number of blood vessels with a radially arranged “spoked wheel” pattern around the implant (asterisk). (B) CAM of 12-day-old chick embryo incubated for 4 days with a sponge absorbed with vehicle alone (PBS), used as negative control. No vascular response is detectable around the sponge (asterisk). (C) Histologic section of a sponge treated with I-309. Note, among the sponge trabeculae, a collagenous matrix containing numerous capillaries (arrowheads) and a mononuclear cell infiltrate. (D) Histologic section of a sponge treated with PBS. No collagenous matrix and blood vessels are detectable among the sponge trabeculae. Original magnifications: A, B, × 50; C, D, × 250.