Effects of aphidicholine and CHX on IFN-α–mediated down-regulation of hTERT levels.

(A) Exponentially growing Daudi cells were cultured in the presence or absence of aphidicholine (1 μmol/L) for 24 hours, and the cells were then further treated with either aphidicholine alone or aphidicholine plus IFN-α for an additional 24 and 48 hours. (B) Exponentially growing U266 cells were cultured in the presence or absence of CHX (50 μg/mL) for 15 minutes, after which the cells were treated with either CHX alone or CHX plus IFN-α for an additional 4 hours. Data shown are representative of 3 independent experiments. C denotes the competitor band.