NKT-cell–mediated control of MCA-induced fibrosarcoma by independent pfp and IFN-γ activities.
(A) Groups of 10 to 43 B6, B6.Jα2810, B6.pfp0, B6.IFN-γ0, or B6 pfp0.IFN-γ0 mice were injected subcutaneously in the hind flank with 100 μg or 25 μg MCA diluted in 0.1 mL corn oil. Mice were observed weekly for tumor development over the course of 50 to 180 days. Tumors larger than 4 mm in diameter and demonstrating progressive growth over 3 weeks were counted as positive. Groups with statistically higher incidence than B6 mice were noted (*P < .01; **P < .05; Fisher exact test). (B) Representative individual fibrosarcomas from groups of 10 mice (above) were compared with a group of B6 mice receiving 400 μg MCA. Tumor size was measured daily with a caliper square as the product of 2 diameters, and results were recorded as the tumor size (cm2). The mean growth rate of tumors was determined from the gradients of individual plots and was normalized against the mean of the B6 (400 μg) group = 1.0. Data are plotted as the mean ± SE, and significant differences to the B6 (400 μg) control were determined by an unpaired t test with Welch correction (*P < .01)
