The SH2 domain of p190 Bcr/Abl is not required for induction of B-lymphoid leukemia in mice.

Kaplan-Meier survival curve for recipients of marrow transduced with p190 BCR/ABL wild-type and the indicated SH2 mutants (panel A, top) and p210 BCR/ABL wild-type and SH2 mutants (panel B, bottom). Several independent transplantation experiments were carried out for each mutant with similar outcome, and the results are combined here. The number of individual mice in each arm is indicated. All animals developed B-lymphoid leukemia that was identical to that previously described,5 except that the majority of p190 ΔSH2-transduced mice had bulky lymphadenopathy at autopsy, with the size of individual lymph nodes from 5 to 12 mm and the combined weight of inguinal, axillary, and cervical nodes often exceeding 1 g (average, 0.65 ± 0.27 g versus 0.27 ± 0.15 g for p190 wild-type). The survival of mice receiving p190 ΔSH2-transduced marrow was significantly longer (P < .0001, Mantel-Cox test), whereas there was no significant difference in survival between p190 wild-type, p190 R552K, and p190 R552L.