IL-13Rα1 expression by individual HRS cells.

Single HRS cells from case 37 were micromanipulated and subjected to polyA PCR as described in “Materials and methods.” PolyA cDNA products from 20 individual HRS cells (lanes 1-20) were amplified by PCR with IL-13Rα1–specific oligonucleotides. The 430-bp PCR product indicates IL-13Rα1 gene expression in 15 of 20 single HRS cells. The presence of IL-13Rα1 cDNA was confirmed by hybridization with an internal oligonucleotide. MW = 100-bp ladder; lanes 1-20: single HRS cells; lane 21: negative control (single HRS cell, no reverse transcriptase); lane 22: blank; lane 23: negative control (H₂0); lane 24: positive control (cDNA from KM-H2 cells).