CLP36 associates with actin stress fibers in endothelial cells.

Double immunofluorescence staining of CLP36 (Bii,Cii,Dii) and F-actin (Bi,Ci,Di). Controls (Ai-ii) for the specific staining of CLP36 were incubated with preimmune serum IgG (Ai) or with His-CLP36 for 1 hour before addition of anti-CLP36 antibody (Aii). Shown are subconfluent migrating endothelial cells (Ai-Bii), resting confluent endothelial cells (Ci-ii), and confluent endothelial cells exposed to thrombin (1 U/mL) for 2 minutes (Di-ii).