Coordinate and reversible translocation of CLP36 and α-actinin-1 to the cytoskeleton of activated platelets.

Platelets were activated for various times with the thrombin-receptor activating peptide TRAP (20 μmol/L). The proteins in the cytoskeleton (CSK) and the membrane cytoskeleton (MSK) were separated by SDS-PAGE and immunoblotted using anti-CLP36, antiactin, and anti–actinin-1 antibodies; wp indicates whole platelet proteins.