Fig. 4.
Effect of anti-GP Ibα monoclonal antibodies on the adhesion of GP Ib-IX–expressing CHO cells to vWF under flow.
Mean velocities are shown for CHOαβIX cells rolling over a glass surface coated with 50 μg/mL vWF (this concentration saturates the vWF binding sites on the glass coverslip) after the application of a shear stress of 10 dynes/cm2. CHOβIX cells, lacking GP Ibα, did not adhere to the surface. Where indicated, CHOαβIX cells were pretreated with monoclonal antibody at 25 μg/mL for 15 minutes. This concentration was well above the saturating concentration for each of these antibodies. Results are the means of analysis of 300 to 700 cells from 3 to 5 experimental runs.
