Flow cytometric analysis of MFT3 antigen expression in T-cell clones and in cells from various lymphoid tissues.

Autoreactive T-cell clones KGU139 (A) and KGU140 (B) derived from B/WF1 mice were stained with MFT3 monoclonal antibody (mAb; IgG2a) followed by fluorescein isothiocyanate conjugated (FITC)–MAR18.5 and analyzed on a fluorescence-activated cell-sorter scanner (FACScan). Cells from the thymus (C), spleen (D), lymph nodes (E), and bone marrow (F) of BALB/c mice were stained and analyzed as above. The shaded histogram shows results from staining with MFT3 mAb; and the open histogram, results from staining without MFT3 mAb.