Western blot of myotube-synthesized and hepatocyte-derived F.IX after enzymatic cleavage by N-glycosidase F.

Western blot of 7.5% SDS-PAGE gel of purified F.IX after digestion with N-glycosidase F using a monoclonal antibody to the heavy chain of F.IX. Myotube-synthesized hF.IX undigested (lane 1), digested (lane 2), HepG2-synthesized F.IX undigested (lane 3), digested (lane 4), and plasma-derived F.IX undigested (lane 5), digested (lane 6).