Fluorophore-assisted carbohydrate electrophoresis (FACE) profiling of the N-linked oligosaccharide.

Oligosaccharides were released by enzymatic digestion with N-glycosidase F from myotube-synthesized F.IX (lane 2) and plasma-derived F.IX (lane 3). Lane 1 contains a ladder of glucose polymers. The position of Glucose 4 (G4) is indicated, and N-linked oligosaccharides migrate in the gel above position G4. N-glycosidase F digestion of trypsin inhibitor serves as a control for enzyme activity (lane 5). A maltotetrose quantitative standard equal to 100 pmol Glucose 4 oligosaccharides was used as control for quantitation (lane 4).