I-309, vMIP-I, and the CM from 6K apo(a) derivative incubated with HUVECs are chemotactic for endothelial cells.

I-309 or vMIP-I (100 ng/mL) was placed in both top and bottom wells, only in the top well (containing the HUVECs), or only in the bottom wells of the chemotaxis chamber. A recombinant apo(a) derivative containing 6 type 4 kringles, kringle V, and the protease domain of apo(a) (40 μg/mL) was incubated 6 hours with HUVECs and the CM (K) was tested for chemotactic activity as described for the purified chemokines. The − indicates medium 199; I, I-309; v, vMIP-I; K, medium from HUVECs treated with 6K apo(a) derivative. Significant activity was observed when the agonist was in the bottom chamber only indicating that the endothelial transmigration was due to chemotaxis and not due to chemokinesis. hpf, high power field.