Polyclonal goat antibody against CCR8 inhibits the chemotaxis of HUVECs in response to I-309 and vMIP-I.

I-309 (▨; 100 ng/mL) and vMIP-I (▪; 100 ng/mL) were tested for endothelial cell chemotactic activity as detailed in “Materials and methods.” Prior to assay, the indicator HUVECs were incubated either with normal goat IgG, goat polyclonal antibody against CCR8 (anti-CCR8), or goat polyclonal antibody against CCR2 (anti-CCR2) (1 μg/mL) for 30 minutes at 37°C. The anti-CCR8 significantly inhibited endothelial chemotaxis as induced by I-309 and vMIP-I (*P < .001). hpf, high power field.