CCR8 is found on the endothelium in human coronary endarterectomy specimens.

Sections of human coronary endarterectomy specimens were stained with polyclonal antibody for CCR8 (A), I-309 (B), murine monoclonal antibody against CCR8 (C), vWF antigen (D), monocyte/macrophage antigen CD-68 (E), and the IgG1 isotype control for the monoclonal antibody against CCR8 (F). Endothelial cells in the lesion, identified by their staining for vWF antigen (D, arrow) stain positively for CCR8 with both polyclonal and monoclonal antibodies (A,C, arrows), and for I-309 (B, arrow). Anti-CD-68 did not stain the endothelium (E, arrow); however, it stained cells in the subendothelial neointima. The murine IgG1 isotype control did not stain the endothelial surface (F, arrow) or the underlying tissue. (DAB-peroxidase, hematoxylin counterstain, original magnification × 400).