Northern blot analysis of laminin chain mRNA expression in human bone marrow stromal cells.

Twenty micrograms of total RNA isolated from the bone marrow–derived stromal cell lines L88/5 (a lanes), L87/4 (b lanes), and HS-5 (c lanes) as well as 10 μg of total RNA isolated from primary stromal cells of LTMCs (d lanes) were hybridized with DIG-labeled cDNA probes corresponding to the human laminin α2, α4, α5, β1, β2, and γ1 chains. The amount of RNA loaded per lane was determined by hybridization with a DIG-labeled cDNA probe for human GAPDH mRNA (←). The size of the detected laminin chain mRNAs was estimated using a DIG-labeled molecular weight marker whose largest fragment runs at 7 kb (⇐). Expression of laminin α4, α5, β1, β2, and γ1 chain mRNAs was found in the stromal cell line HS-5 (lane c) and the primary bone marrow stromal cells (lane d), whereas the stromal cell lines L88/5 and L87/4 express mainly laminin α5, β1, β2, and γ1 chain mRNAs. No expression of α2 chain mRNA was detected in any of the analyzed stromal cells.