Cell attachment to different laminin preparations.

The CD34⁺ hematopoietic cell line KG1a was used to analyze adhesive interactions of the laminin isoforms LN-1, LN-2/4, and LN-10/11 in a conventional cell adhesion assay where the different laminin preparations were immobilized onto small areas of Petri dishes. LN-1 showed no cell adhesive capacity, even at a concentration of 100 μg/mL. A specific interaction of LN-2/4 with KG1a cells (represented by the white dots) was detected at 100 μg/mL, but at lower concentrations (eg, 10 μg/mL) no cell attachment to LN-2/4 was observed. LN-10/11, on the contrary, showed a strong adhesive activity at 10 μg/mL. Even at 1.0 μg/mL LN-10/11, cell attachment was only marginally diminished, and at 0.1 μg/mL no cell attachment could be observed, indicating concentration-dependent attachment. (Original magnification × 30.)