Aggregation response of platelets to chemokines alone and in combination as well as in the presence of heparin, low-molecular-weight heparin (Fragmin), heparinase, or chondroitinases.

Upper series of aggregation profiles shows washed platelets activated with 100 nmol/L TARC, from left, control, in the presence of 1 U/mL, 5 U/mL, and 20 U/mL heparin, respectively. Middle series shows washed platelets activated with 100 nmol/L TARC, from left, in the presence of 1 U/mL, 5 U/mL, and 20 U/mL Fragmin, respectively. Lower series shows washed platelets activated with 100 nmol/L TARC, from left, control, after incubation for 30 minutes at 37°C with 5 U/mL heparinases I and III, 5 U/mL chondoitinases ABC, and 5 U/mL heparitinase I and III plus 5 U/mL chondoitinase ABC, respectively. These results are typical of at least 3 similar experiments.