Representative analysis of transcripts found in different populations of primitive adult BM cells.

RNA extracts from purified CD34⁺CD38⁻(34⁺38⁻) and CD34⁺CD38⁺ (34⁺38⁺) adult BM cells and their progeny obtained after 5 days of stimulation by 2 growth factor combinations (A and B, described in “Materials and methods”) were subjected to semiquantitative RT-PCR, followed by Southern blot analysis using gene-specific cDNA probes. Each filter was then stripped and reprobed with a GAPDH probe (not shown) to allow normalization for cDNA loading.