Analysis of changes in gene expression in growth factor-stimulated adult BM CD34⁺ cells remaining quiescent for 40 hours.

Panel A shows representative blots comparing transcript cDNAs for 9 genes expressed in freshly isolated CD34⁺ G₀(G₀i) cells, cells found to be still in G₀after 40 hours in culture (G₀′ cells), and the remaining (NG₀′) cells. Cells were cultured and isolated as described in Figure 5 and then RNA extracts were prepared and subjected to semiquantitative RT-PCR analysis as described in the “Materials and methods.” Panel B shows the same data quantitated and normalized as in Figure 2, but using the G₀i cells as the reference population in this case (value for each transcript = 1.0 for the G₀i cells). Shown are the mean ± SEM of data from 3 independent experiments in each of which BM cells from a different individual were used. Values found to be significantly different from 1.0 (P < .05) are indicated by an asterisk (*).