Fig. 5.
![Fig. 5. Released BLyS induces B-cell proliferation and IgG production. / (A) [3H]thymidine incorporation by B cells was assessed after a 4-day culture with serial dilutions of U937-conditioned medium (indicated by a closed circle) in presence of the neutralizing mAb 15C10 (indicated by an open circle) or an unrelated mAb of the same isotype (indicated by closed triangles). Anti-IgM was added to the culture as costimulus. [3H]thymidine incorporation of B cells in the presence of anti-IgM was 630 dpm. (B) Purified B cells were cultured for 10 days with SAC and serial dilutions of the U937-conditioned medium in the absence or presence of mAb 15C10. IgG production was evaluated by ELISA.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/1/10.1182_blood.v97.1.198/6/h80110564005.jpeg?Expires=1735843170&Signature=Z~JU0CldzcJ6jhKm6eG6OHfX7aPSMI9~votXtD2URPanh2VJ01Q3InvgwM~Vu0FtzhYX4LY11OY8ehM6YAQVCp3BtH~zhLes63TgB9RIrA5zCAmKeY0lCcsTjUdZF~Tmp2R4Y5qkFC6DU3pbT82RRzuWZK1hxT2N63cn28RuDO2HxfO3e09eLCIyNgl5uhVQikBXSLEn0ecINhgm-LPTVvX8NmcPGYEQWuc8la7Y8OBxFR-HlnkXWuNqJlSYYAiru8j6wYPGdqwqfM67xnQ0uUOxouEsxtXSGFtdHCGcSB9dtJvLOme0VsCLV7RAgtVPRnL63xx0igZxG91F5UVXcQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Released BLyS induces B-cell proliferation and IgG production.
(A) [3H]thymidine incorporation by B cells was assessed after a 4-day culture with serial dilutions of U937-conditioned medium (indicated by a closed circle) in presence of the neutralizing mAb 15C10 (indicated by an open circle) or an unrelated mAb of the same isotype (indicated by closed triangles). Anti-IgM was added to the culture as costimulus. [3H]thymidine incorporation of B cells in the presence of anti-IgM was 630 dpm. (B) Purified B cells were cultured for 10 days with SAC and serial dilutions of the U937-conditioned medium in the absence or presence of mAb 15C10. IgG production was evaluated by ELISA.
