Fig. 1.
Cell proliferation assays.
Overexpression of E2F-1 confers cytokine growth factor independence to the IL-3–dependent cell line BaF-B03. Proliferation was assessed at the indicated times by staining with trypan blue. (A) Cells were cultured with or without IL-3. (B) Clones of E2F-1–overexpressing cells were cultured without IL-3.