Fig. 2.
Tyrosine phosphorylation in platelets after TPO stimulation.
Platelets of patient CAMT-11 and of a healthy donor were preincubated with 2.7 μg/mL rhTPO before lysis and Western blot analysis of tyrosine-phosphorylated proteins (A). Phosphorylation of Jak2 was proved after immunoprecipitation with anti-Jak2 and Western blot detection of tyrosine-phosphorylated proteins and Jak2 (B).