FACS profiles of the cell populations tested.

(A) Dot plot of CD34-FITC versus CD38-PE–labeled PI⁻cells showing CD34⁺38⁻, CD34⁺CD38⁺, and CD34⁻ populations. (B) Dot plot of Hst-stained versus Py-stained PI⁻CD34⁺ cells showing CD34⁺ G₀ (R1) and CD34⁺ G₁/S/G₂/M (R2) populations. (C) Histogram of CFSE-stained PI⁻CD34⁺ cells showing TPO-resistant (M1) and TPO-responsive (M2) populations.