The proximal promoter controls

CD13/APN transcription in HUVECs and endothelial cell lines. (A) Plasmids containing the proximal or distal promoter fragments immediately upstream of the luciferase reporter gene were transiently transfected into primary HUVECs. Lysates of triplicate dishes were assayed at 24 hours for luciferase activity. pGL2basic refers to the promoterless control luciferase plasmid. (B) The identical experiment in KS1767 cells using CAT reporter plasmids. pEMSV-CAT refers to the positive control plasmid and pCAT3M to the promoterless control plasmid. Equal amounts of lysate (75 μg) were assayed in each lane.