Cbl is tyrosine-phosphorylated and associates with PI-3 kinase, Crk-L, and 14-3-3β upon SDF-1α stimulation.

Lysates obtained from Jurkat cells were unstimulated (0) or stimulated with 100 ng/mL SDF-1α for various time periods and then lysed. The cell lysates were immunoprecipitated with anti-cbl antibody run on SDS-PAGE and immunoblotted with antiphosphotyrosine antibody (A, top panel), followed by anti-cbl antibody (A, bottom panel). Equivalent amounts of cell lysate (500 μg) were immunoprecipitated with anti-cbl (B,D,F), anti–PI-3 kinase (C), or anti-Crk-L (E) and then run on SDS-PAGE and immunoblotted with anti–PI-3 kinase (B), anti-cbl (C,E), anti–Crk-L (D), or anti–14-3-3β (F). C represents the antibody control, and TCL is 50 μL of total cell lysate.